• Sites:
• K114
• K159
• K162
• S195
• D199
• S200
• K240

Entry	Protein	Modification	Peptide Sequence	Mod Site/Crystal Structure	Mass spectrum	Chemical Formula	Exact Mass (Da)	Description
TK114-a	Trypsin	SFABP-Lys	LKSAASLNSR	K114	MSMS	C14H15N1O3S	277.08	Labelling of Lysine 114 by the SFABP.
TK114-b	Trypsin	SFABP-Lys	LKSAASLNSR	K114	MSMS	C14H15N1O3S	277.08	Labelling of Trypsin's Lysine 114 by the SFABP, in a HeLa nuclear extract spiked with bovine trypsin (5-fold excess (w/w) HeLa nuclear proteins).
TK114-c	Trypsin	SFABP-Lys	LKSAASLNSR	K114	MSMS	C14H15N1O3S	277.08	Labelling of Trypsin's Lysine 114 by the SFABP, in a HeLa nuclear extract spiked with bovine trypsin (20-fold excess (w/w) HeLa nuclear proteins).
TK159	Trypsin	SFABP-Lys	SSGTSYPDVLKCLK	K159	MSMS	C14H15N1O3S	277.08	Labelling of Lysine 159 by the SFABP. Mass discrepancy between observed and expected adducts is due to selection of the +2 Da isotope for fragmentation by the instrument.
TK162-a	Trypsin	SFABP-Lys	CLKAPILSDSSCK	K162	MSMS	C14H15N1O3S	277.08	Labelling of Lysine 162 by the SFABP.
TK162-b	Trypsin	SFABP-Lys	CLKAPILSDSSCK	K162	MSMS	C14H15N1O3S	277.08	Labelling of Trypsin's Lysine 162 by the SFABP, in a HeLa nuclear extract spiked with bovine trypsin (5-fold excess (w/w) HeLa nuclear proteins).
TK162-c	Trypsin	SFABP-Lys	CLKAPILSDSSCK	K162	MSMS	C14H15N1O3S	277.08	Labelling of Trypsin's Lysine 162 by the SFABP, in a HeLa nuclear extract spiked with bovine trypsin (20-fold excess (w/w) HeLa nuclear proteins).
TS195	Trypsin	AEBSF-Ser	DSCQGDSGGPVVCSGK	S195	MSMS	C8H9N1O2S	183.04	Labelling of trypsin's S195 by AEBSF.
TD199	Trypsin	N/A	DSCQGDSGGPVVCSGK	D199	MSMS	18O	18.0	Labelling of Trypsin's Aspartic Acid 199 by a heavy oxygen.
TS200-a	Trypsin	DTT/IA-Ser	DSCQGDSGGPVVCSGK	S200	MSMS	C6H11N1O2S2	193.02	Labelling of Trypsin's active site Serine 200 by the SFABP, followed by displacement of the sulfonate by DTT and its capping by IA.
TS200-b	Trypsin	DTT/NEM-Ser	DSCQGDSGGPVVCSGK	S200	MSMS	C10H15N1O3S2	261.05	Labelling of Trypsin's active site Serine 200 by the SFABP, followed by displacement of the sulfonate by DTT and its capping by NEM.
TS200-c	Trypsin	Thiophenol-Ser	DSCQGDSGGPVVCSGK	S200	MS1 / MSMS	C6H379Br1O-1S1	169.92	Labelling of Trypsin's active site Serine 200 by the SFABP, followed by displacement of the sulfonate by 3-bromothiophenol. MS/MS of the light isotope (M/Z = 890.34).
TS200-d	Trypsin	Thiophenol-Ser	DSCQGDSGGPVVCSGK	S200	MS1 / MSMS	C6H381Br1O-1S1	171.92	Labelling of Trypsin's active site Serine 200 by the SFABP, followed by displacement of the sulfonate by 3-bromothiophenol. MS/MS of the heavy isotope (M/Z = 891.34).
TS200-e	Trypsin	Thiophenol-Ser	DSCQGDSGGPVVCSGK	S200	MS1 / MSMS	C6H381Br1O-1S1	171.92	Labelling of Trypsin's active site Serine 200 by the SFABP, followed by displacement of the sulfonate by 3-bromothiophenol, in a HeLa nuclear extract spiked with bovine trypsin (34-fold excess (w/w) HeLa nuclear proteins). MS/MS of the heavy isotope (M/Z = 891.34).
TS200-f	Trypsin	DTT/IA-AEBSF	DSCQGDSGGPVVCSGK	S200	MSMS	C6H11N1O2S2	193.02	Labelling of Trypsin's active site Serine 200 by AEBSF, followed by displacement of the sulfonate by DTT and its capping by IA.
TS200-g	Trypsin	Thiophenol-Ser-AEBSF	DSCQGDSGGPVVCSGK	S200	MSMS	C6H379Br1O-1S1	169.92	Labelling of Trypsin's active site Serine 200 by AEBSF, followed by displacement of the sulfonate by 3-bromothiophenol. MS/MS of the light isotope (M/Z = 890.34).
TS200-h	Trypsin	Thiophenol-Ser-AEBSF	DSCQGDSGGPVVCSGK	S200	MSMS	C6H381Br1O-1S1	171.92	Labelling of Trypsin's active site Serine 200 by AEBSF, followed by displacement of the sulfonate by 3-bromothiophenol. MS/MS of the heavy isotope (M/Z = 891.34).
TS200-i	Trypsin	Thiophenol-Ser-AEBSF	DSCQGDSGGPVVCSGK	S200	MSMS	C6H381Br1O-1S1	171.92	Labelling of Trypsin's active site Serine 200 by AEBSF, followed by displacement of the sulfonate by 3-bromothiophenol, in a HeLa nuclear extract spiked with bovine trypsin. MS/MS of the heavy isotope (M/Z = 891.34).
TK240-a	Trypsin	SFABP-Lys	VCNYVSWIKQTIASN	K240	MSMS	C14H15N1O3S	277.08	Labelling of Lysine 240 by the SFABP.
TK240-b	Trypsin	SFABP-Lys	VCNYVSWIKQTIASN	K240	MSMS	C14H15N1O3S	277.08	Labelling of Trypsin's Lysine 240 by the SFABP, in a HeLa nuclear extract spiked with bovine trypsin (5-fold excess (w/w) HeLa nuclear proteins).